Customization: | Available |
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CAS No.: | None |
Formula: | None |
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Description
Ni GK® FF (IDA) is a new type of affinity chromatography media independently developed by Sunresin. It is formed by binding iminodiacetic acid groups to fast flow agarose gel filtration chromatography media and then chelating metal ions Ni2+. Ni GK® FF (IDA) has good chemical stability and biocompatibility,
Ni GK® FF (IDA) uses the affinity adsorption of histidine in sample components and metal ions for separation and purification, and can be used for separation and purification of polypeptides, proteins, nucleotides, phosphorylated proteins and recombinant protein with His tag.
Properties
Product | Ni GK® FF (IDA) |
Appearance | Spherical gel, odorless and tasteless |
Matrix | GK® 6FF |
Max. flow rate (cm/h) | ≥370 |
Pressure resistance (MPa) | 0.3 |
Particle size (μm) | 45-165 |
Ion binding capacity | ~30(Ni2+ umol/ml) |
pH stability | 3~13 (operational), 2~14 (CIP) |
Chemical Stability | Stable in common aqueous solutions: 0.1M sodium hydroxide; 8M urea (24h); 6M Guanidine Hydrochloride (24h), 1M NaOH (24h), 5mm DTT (24h) |
Application | Purification of recombinant proteins with His tag |
Storage
Sealed and stored at 4~30°C (preservation solution is 20% ethanol) in a ventilated, dry and clean place, do not freeze. Used media should be stored at 4-8°C in 20% ethanol solution.
Transportation
Avoid sunlight, rain, and heavy pressure during transportation. It is strictly forbidden to transport with toxic and hazardous materials
1. Top quality materials with competitive price;
2. Large capacity of stable materials supply;
3. Safety and fast shipment by express/air/sea;
4. Sample order acceptable;
5. Professional and great packing as customized;
6. Convenient payment terms by T/T, Credit Card and online order;
7. Reply inquiries in time
Q: Who are you?
A: We are a professional and with more than 20 years experienced supplier and exporter for Industrial & fine chemicals, APIs, Reagent & Lab Supplies, and Food & Food Additives in China.
Q: How can you guarantee quality?
A: We could provide pre-production sample before mass production. And we also accept the final inspection(third-party) before shipment.
Q: Do you supply free samples?
A: Generally, we could provide free sample for quality evaluation, but the international shipping cost should be undertook by customers.
Q: Which payment terms do you accept?
A: We could accept the payment through T/T, L/C and Online payment.
Q: Can I pay with a credit card
A: Yes, you could feel free to make your payment through credit card. Please contact us for more details